nifZ [2018-02-12T11:58:47.000Z]

cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA

Locus: BSU29590

Isoelectric point: 6.34

Molecular weight: 41.33 kDa

Protein length: 381 aa

Gene length: 1143 bp

Function: biosynthesis of 4-thiouridine in tRNA

Product: cysteine desulfurase

Essential: no

Synonyms: iscSB

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 3,026,957 → 3,028,102

The protein

Catalyzed reaction/ biological activity

• removes sulfuryl group from cysteine and transfers it to ThiI PubMed

Paralogous protein(s)

• NifS, YrvO, SufS, YcbU

Structure

• 1EG5 (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) PubMed

Expression and Regulation

Operons

• Genes: nifZ-thiI-sspA
  Description: PubMed

Biological materials

Mutant

• BKE29590 (ΔnifZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTA
• BKK29590 (ΔnifZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTA

References

Reviews

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Original publications

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